Trainee

Supervisor

Publications

William Waas

Dalby

·  Waas WF, Dalby KN (2001). Purification of a model substrate for transcription factor phosphorylation by ERK2. Protein Expr Purif 23:191-7.

·  Waas WF, Lo HH, Dalby KN. The kinetic mechanism of the dual phosphorylation of the ATF2 transcription factor by p38 mitogen-activated protein (MAP) kinase alpha. Implications for signal/response profiles of MAP kinase pathways. J Biol Chem 276:5676-84.

·  Waas WF, Dalby KN (2002). Transient protein-protein interactions and a random-ordered kinetic mechanism for the phosphorylation of a transcription factor by extracellular regulated protein kinase 2. J Biol Chem Jan 25 [electronic publication ahead of print]

·  Waas WF, Dalby KN. The magnesium dependence of Extracellular- signal Regulated Kinase-2 (ERK2) : Requirement of a second divalent cation for catalysis. Submitted to J Biol Chem

·  Waas WF, Rainey M, Dalby KN. Extracellular-Signal Regulated Kinase-2 (ERK2): A Transient Kinetic Analysis. Manuscript in Preparation.

Danielle Tullman

Georgiou

Kishore Doshi

Gutell

Wendi Wagner

Robertus

Karl Griswold

Iverson

Ron Lafond

Georgiou

·  Lafond R, Zhan X, Georgiou G (2002) Screening and Selection Strategies for Disulfide Isomerase Activity. Methods in Enzymology (in press)

·  Samuelson P, Lafond R, Georgiou G (2002) Directed evolution of a disulfide isomerase using phage linked assisted in vivo folding: PLAinFold (in preparation)

J. Daniel Piper

Kitto

·  Piper, J. D., Caras, J. W., Stephens, N. A., Kurien, A., Fisher, J., Prasannan, P., Donnelly, J. A., Kitto, G. B. 2002. A b2-microglobulin viral epitope fusion protein vaccine provides protection against a lethal viral challenge. In preparation.

·  Piper, J. D., Donnelly, J. A., Beck, S., Watson, L., Kitto, G. B. 2002. A novel DNA vaccine that elicits a strong CTL response and provides protection against a viral challenge. In preparation.

·  Piper, J. D., Grubbs, B. H., Kitto, G. B. 2002. A Salmonella based vaccine provides protection against a lethal Sendai challenge. In preparation.

·  Gillis, H. P., Choutov, D. A., Steiner IV, P. A., Piper, J. D., Crouch, J. H., Dove, P. M., Martin, K. P. 1995. Low energy electron-enhanced etching of Si (100) in hydrogen/helium direct-current plasma. Appl. Phys. Let. 66 (19): 2475-2477.

Jennie Baier (Leach)

Schmidt

·  Schmidt CE, Baier JM (2000). Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering. Biomaterials 21:2215-31.

·  Fischbeck JA, Baier JM, R. Akella, Hern-Anderson D, Schmidt CE (2001). Genetic modification of alphaGal expression in xenogeneic endothelial cells yields complex immunological response. Tissue Eng 7:743-56.

·  Leach JB, Bivens KA, Patrick CW, Schmidt CE (accepted). Photocrosslinked hyaluronic acid hydrogels: natural biodegradable tissue engineering scaffolds. Biotechnology and Bioengineering.

·  Schmidt CE, Leach JB, Shoichet MS. Neural Tissue Engineering: Strategies for Repair and Regeneration. Annual Reviews in Biomedical Engineering. Manuscript in Preparation.

·  Leach JB, Schmidt CE. Hyaluronic Acid. In: G.E. Wnek, G.L. Bowlin, ed. Encyclopedia of Biomaterials and Biomedical Engineering, Marcel Dekker. Manuscript in Preparation.

Michael A. Siano

Harshey

·  Toguchi A, Siano M, Burkart M, Harshey RM (2000.) Genetics of swarming motility in Salmonella typhimurium: Critical role for lipopolysaccharide. J Bacteriol 182: 6308-21.

Meredith M. Murr

Iverson

·  Murr MM, Harting MT, Guelev V, Ren J, Chaires JB, Iverson BL (2001). An octakis-intercalating molecule. Bioorg Med Chem. 9:1141-8.

·  Guelev VM, Cubberley MS, Murr MM, Lokey RS, Iverson BL (2001). Design, synthesis, and characterization of polyintercalating ligands. Methods Enzymol 2340:556-70.

Michael E. Moak

Molineux

·  Moak M, Molineux IJ (2000). Role of the Gp16 lytic transglycosylase motif in bacteriophage T7 virions at the initiation of infection. Mol Microbiol 37:345-55.

Terry Hudson

Schmidt

·  Hudson TW, Evans GRD, Schmidt CE (1999). Engineering Strategies for Peripheral Nerve Repair. Clinics in Plastic Surgery 26: 617-28.

·  Hudson TW, Evans GRD, Schmidt CE (2000). Engineering Strategies for Peripheral Nerve Repair. Orthopedic Clinics of North America 31(3):485-497. Reprinted from: Clinics in Plastic Surgery

·  Collier JH, Camp JP, Hudson TW, Schmidt CE (2000). Synthesis and characterization of polypyrrole/hyaluronic acid composite biomaterials for tissue engineering. J. Biomed. Mater. Res.50:574-584.

·  Rivers TJ, Hudson TW, Schmidt CE (2002). Synthesis of a Novel, Biodegradable Electrically Conducting Polymer for Biomedical Applications. Advanced Functional Materials 12:33-37.

·  Schmidt C, Rivers T, Hudson T, Collier J (in press). Modification of Electroactive Biomaterials for Neural Engineering Applications. Electrifi ed Polymer/Solution Interfaces, ACS Symposium Series.

·  Hudson TW, Liu S, Schmidt CE. Development of a Novel Acellular Peripheral Nerve Graft with a Highly Preserved Extracellular Structure. Manuscript in Preparation.

Akin Halit Davulcu

Anslyn

Keith Friedman

Heller

·  Chen T, Friedman KA, Lei I, Heller A (2000). In Situ Assembled Mass-Transport Controlling Micromembranes and Their Application in Implanted Amperometric Glucose Sensors. Analytical Chemistry 72:3757-63.

·  Heller A, Chen T, Friedman KA (2001). Electrodes with multilayer membranes and methods of using and making the electrodes. PCT Int Appl pp. 36.

·  Friedman KA, Heller A (2001). On the non-uniform distribution of guanine in introns of human genes: possible protection of exons against oxidation by proximal intron poly-G sequences. J Phys Chem B 105:11859-65.

Paul Bessette

Georgiou

·  Rietsch A, Bessette P, Georgiou G, Beckwith, J (1997). Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J Bacteriol 179: 6602-8.

·  Bessette PH, Cotto JJ, Gilbert HF, Georgiou G (1999). In vivo and in vitro function of the Escherichia coli periplasmic cysteine oxidoreductase DsbG. J Biol Chem 274(12): 7784-92.

·  Bessette PH, Åslund F, Beckwith J, Georgiou G (1999). Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc Natl Acad Sci USA 96:13703-8.

·  Åslund F, Bessette P, Georgiou G, Beckwith J (1999). Efficient production of disulfide bonded proteins in the cytoplasm in Œoxidizing‚ mutants of E. coli inNovations (Novagen) 10:10-11.

·  Bessette PH, Qiu J, Swartz JR, Bardwell JCA, Georgiou G (2001). Effects of the sequences of the active-site dipeptides of DsbA and DsbC on in vivo folding of multi-disulfide proteins in Escherichia coli. J Bacteriol 183:980-8.

Tyrell Rivers

Schmidt

·  Rivers TJ, Hudson TW, Schmidt CE (2002). Synthesis of a Novel, Biodegradable Electrically Conducting Polymer for Biomedical Applications. Advanced Functional Materials 12:33-37.

·  Schmidt C, Rivers T, Hudson T, Collier J (in press). Modification of Electroactive Biomaterials for Neural Engineering Applications. Electrified Polymer/Solution Interfaces, ACS Symposium Series.

Michael Wallace

Iverson

·  Wallace MB, Iverson B (1996). The Influence of Hapten Size on the Catalytic Activity of Elicited Polyclonal Antibodies J. Am. Chem. Soc. 118:251-252.

·  Dragovich PS, Webber SE, Babine RE, Fuhrman SA, Patick AK, Matthews DA, Lee CA, Reich SH, Prins TJ, Marakovits JT, Littlefield ES, Zhou R, Tikhe J, Ford CE, Wallace MB, Meador JW III, Ferre RA, Brown EL, Binford SL, Harr JEV, DeLisle DM, Worland ST (1998). Structure-Based Design, Synthesis, and Biological Evaluation of Irreversible Human Rhinovirus 3C Protease Inhibitors. 1. Michael Acceptor Structure-Activity Studies. J Med Chem 41:2806-18.

·  Dragovich PS, Webber SE, Babine RE, Fuhrman SA, Patick AK, Matthews DA, Reich SH, Marakovits JT, Prins TJ, Zhou R, Tikhe J, Littlefield ES, Bleckman TM, Wallace MB, Little TL, Ford CE, Meador JW III, Ferre RA, Brown EL, Binford SL, DeLisle DM, Worland ST (1998). Structure-Based Design, Synthesis, and Biological Evaluation of Irreversible Human Rhinovirus 3C Protease Inhibitors. 2. Peptide Structure-Activity Studies. J Med Chem 41:2819-34.

·  Reich SH, Johnson T, Wallace MB, Kephart SE, Fuhrman SA, Worland ST, Matthews DA, Hendrickson TF, Chan F, Meador J III, Ferre RA, Brown EL, DeLisle DM, Patick AK, Binford SL, Ford CE (2000). Substituted Benzamide Inhibitors of Human Rhinovirus 3C Protease: Structure-Based Design, Synthesis, and Biological Evaluation. J Med Chem 43:1670-83.

Anne Marie Murphy

Kodadek

·  Zhang Z, Murphy A, Hu JC, Kodadek T (1999). Genetic selection of short peptides that support protein oligomerization in vivo. Curr Biol 9:417-20.

Patrick Daugherty

Georgiou

·  Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL, Curtiss III, R (1997). Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines. Nature Biotechnology 15:29-34.

·  Daugherty PS, Chen G, Olsen MJ, Iverson BL, Georgiou G (1998). Antibody affinity maturation using bacterial surface display. Protein Eng 11:825-32.

·  Daugherty PS, Olsen MJ, Iverson BL, Georgiou G (1998). Development of an optimized expression system for the screening of antibody libraries displayed on the E.coli surface. Protein Eng 12:613-21.

·  Olsen MJ, Stephens DL, Griffiths D, Daugherty PS, Georgiou G, Iverson BL (2000). Function-based isolation of novel enzymes from a large library. Nature Biotechnology 18:1071-1074.

·  Daugherty PS, Iverson BL, Georgiou G (2000) Flow cytometric screening of cell-based libraries. J Immun Method 243:211-27.

·  Daugherty PS, Chen G, Iverson BL, Georgiou G (2000). Quantitative analysis of the effect of mutation frequency on the affinity maturation of single chain Fv antibodies. Proc Nat Acad Sci 97:2029-34.

·  Daugherty PS (in press). Cell surface display and cytometric screening for protein ligand isolation and engineering. In: Vector Targeting for Therapeutic Gene Delivery, Wiley Press.

Maha Zewail-Foote

Hurley

·  Zewail-Foote M, Hurley L (1999). Molecular Approaches to Achieving Control of Gene Expression by Drug Intervention at the Transcriptional Level. Anti-Cancer Drug Design 14:1-9.

·  Zewail-Foote M, Hurley LH (1999). Ecteinascidin 743: A Minor Groove Alkylator that Bends DNA toward the Major Groove. J Medicinal Chemistry 42:2493-7.

·  Zewail-Foote M, Ven-Shun L, Kohn H, Bearss D, Guzman M, Hurley LH (2001). The inefficiency of incisions of ecteinascidin 743-DNA adducts by the UvrABC nuclease and the unique structural feature of the DNA adducts can be used to explain the repair-dependent toxicities of this antitumor agent. Chemistry and Biology 8:1033-49.

·  Zewail-Foote M, Hurley LH (2001). Differential rates of reversibility of Ecteinascidin 743-DNA covalent adducts from different sequences lead to migration to favored bonding sites. J Am Chem Soc 123:6485-95.

·  Zewail-Foote M, Hurley LH (2001). Ecteinascidin 743: Characterization of Its Covalent DNA Adducts and Chemical Stability. The Antitumor Agent In Snyder, R. e. al., ed. Biological Reactive Intermediates VI. New York, Kluwer Academic/Plenum.

Mary Susan Burnett

Kitto

·   Burnett MS, Wang N, Hofmann M, Kitto BG (2000). Potential live vaccines for HIV. Vaccine 19:735-42.

·   Epstein SE, Zhu J, Burnett MS, Zhou YF, Vercellotti G, Hajjar D (2000). Infection and atherosclerosis: potential roles of pathogen burden and molecular mimicry. Arterioscler Thromb Vasc Biol 20:1417-20.

·   Burnett MS, Gaydos CA, Madico GE, Glad SM, Paigen B, Quinn TC, Epstein SE (2001). Atherosclerosis in apoE knockout mice infected with multiple pathogens. J Infect Dis 183:226-31.

·   Hsich E, Zhou YF, Paigen B, Johnson TM, Burnett MS, Epstein SE (2001). Cytomegalovirus infection increases development of atherosclerosis in Apolipoprotein-E knockout mice. Atherosclerosis 156:23-8.

·  Serum of cytomegalovirus-infected mice induces monocyte chemoattractant protein-1 expression by endothelial cells (2001). Rott D, Zhu J, Burnett MS, Zhou YF, Wasserman A, Walker J, Epstein SE. J Infect Dis 184:1109-13.