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Seminars
Single Cell Transcriptional Profiling by Sequential FISH
3:30 pm
Location: BME 3.204
Speaker: Long Cai, PhD
Assistant Professor of Chemistry
California Institute of Technology
We have recently demonstrated a technology using sequential hybridization and single molecule FISH to multiplex a large number of mRNA molecules directly in single cells in complex tissue samples. We call this method seqFISH and it is conceptually akin to “sequencing” mRNAs directly in cells by FISH. My lab is applying this technology to profile 100 or more genes directly in situ. Using seqFISH, we will be able to understand the combinatorial expression patterns in single cells that would provide molecular identifier or “barcodes” for cell spatially heterogeneous samples.
Host: Jenny Jiang, PhD