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Abstract:

Cell biological research relies heavily on the capabilities of light microscopes to resolve structures or processes of interest at the nanoscale. My research group is developing super-resolution technology which achieve down to about 20 nm resolution in 3D and applies them to cell biological questions. We are, in particular, focused on improving the 3D resolution, speed, live-cell compatibility, throughput and depth penetration of STED and PALM/STORM microscopes. In my presentation, I will provide an overview of recent developments in my lab relevant for cell biological research. I will highlight our recent development of multicolor 4Pi-SMS imaging at 20 nm 3D resolution and live-cell applications of STED microscopy to investigate nanoscale dynamics of the endoplasmic reticulum. I declare financial interests in Bruker Corp. and Hamamatsu Photonics.

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